FastQCFastQC Report
Tue 27 Aug 2019
H3JVYBGXC_n01_0730-5R-EV.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3JVYBGXC_n01_0730-5R-EV.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19062555
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT406270.21312463098467124No Hit
CCCGACTGTCCCTGTTAATCATTACTCCGATCCCGAAGGCCAACACAATA231950.12167833745266571No Hit
CGAATGGGGAGCCCACAGGCCGACGCCCGGAGCACGCTGATGCCGAGGCA200050.10494395950595291No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCTA115850.036.7349664
GTACTCT116500.036.3196263
TCTAGTT127750.033.3390777
ACCTACT114450.027.6803633
ACACCTA117500.027.01909331
GTTACCT158700.026.28585214
CTAATGC157950.026.12496221
TAGTTGT163500.025.8140939
TGTTACC162350.025.75948713
ATGCTGG160250.025.68555624
TGGACAC157300.025.63370728
TACCTCT164700.025.49831616
TCAGGAC118750.025.43393560
TCTAATG165600.025.0227920
TAATGCT167350.024.6368122
GACTATC125650.024.23196455
AATGCTG172350.024.00343123
TATCAGG132050.023.13715658
TGTACTC186700.022.5133862
TGCTGGA182600.022.42672725
Produced by FastQC (version 0.11.5)