FastQCFastQC Report
Tue 27 Aug 2019
H3JVYBGXC_n01_0730-4R-FBH3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3JVYBGXC_n01_0730-4R-FBH3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18504057
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT272240.14712449275312978No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCTA106950.026.931984
GTACTCT109800.025.5952243
ACTCTAG141600.023.8760835
TCTAGTT122150.023.8660667
ACCTACT92600.022.68100433
ACACCTA99550.021.20120231
CTAATGC129800.021.1132521
GTTACCT133950.020.74480614
CTCTAGT149300.020.5584416
TGTACTC140150.020.5521452
ATGCTGG135700.019.55121624
GACTATC102300.019.46614655
TACCTCT142900.019.39650216
CTAGTTG145550.019.3802918
TTGTACT144300.019.0443611
TGTTACC146950.018.9572913
TGGACAC140500.018.85795628
TAGTTGT153250.018.8175989
GGACACC137650.018.76538829
CTGTAGG108900.018.70497949
Produced by FastQC (version 0.11.5)