FastQCFastQC Report
Tue 27 Aug 2019
H3JVYBGXC_n01_0730-4G-ABF1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3JVYBGXC_n01_0730-4G-ABF1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34362229
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCTA96450.017.744054
TCTAGTT110950.016.1183097
CTAGCAG106950.015.6415118
GTACTCT108050.015.4826563
CGCTATG94650.015.27266129
CAGGGGA93000.015.2450031
GACTGTC139500.015.0530044
ACCTACT78250.014.62863233
TAGCCCG80500.014.61121134
CGGGCGC80750.014.56192715
ACTCTAG260900.014.52788455
CTAATGC111350.014.49039421
GTCGCTA102150.014.39150827
CGGCAAT106300.013.93036651
GGCGGGC86350.013.77976613
ATGCTGG121400.013.521934524
CGTTCAG86900.013.49142657
ACACCTA88200.013.17547131
GGCGCAT90000.013.14324517
GCCTGGC83600.013.10283316
Produced by FastQC (version 0.11.5)