FastQCFastQC Report
Tue 27 Aug 2019
H3JVYBGXC_n01_0730-3R-HSFA6A.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3JVYBGXC_n01_0730-3R-HSFA6A.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18672991
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT331740.17765766609109382No Hit
CCCGACTGTCCCTGTTAATCATTACTCCGATCCCGAAGGCCAACACAATA283560.15185569360580745No Hit
CGAATGGGGAGCCCACAGGCCGACGCCCGGAGCACGCTGATGCCGAGGCA205560.11008413167445964No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA198200.1061426099332453No Hit
CGGCAATCGGGCGGCGGGCGCATGCGTCGCTTCTAGCCCGGATTCTGACT187760.10055164702858799No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTCT89500.038.830573
TACTCTA91950.038.214724
TCTAGTT104350.033.7389077
ACCTACT87650.029.27408833
ACACCTA91250.028.00152831
GTTACCT126750.027.22459214
TAGTTGT128600.027.1870929
ATGCTGG125300.026.84425224
CTAATGC128200.026.64539121
TACCTCT130100.026.46942116
TCAGGAC93200.026.24833960
TGTTACC133250.025.8177713
TGGACAC129200.025.81700328
GACTATC97200.025.7797855
TCTAATG136200.025.07911120
TAATGCT137250.024.8373822
AATGCTG139200.024.43941723
TATCAGG102700.024.16082258
TGCTGGA143300.023.59451525
ACTATCA107850.023.52630656
Produced by FastQC (version 0.11.5)