FastQCFastQC Report
Tue 27 Aug 2019
H3JVYBGXC_n01_0730-3G-ZAT6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3JVYBGXC_n01_0730-3G-ZAT6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18906792
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT1272800.6731972298632153No Hit
CTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGAC465810.24637178004602792No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA443850.2347569064069674No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA254750.13473993895950195No Hit
CTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAG230800.12207253351070875No Hit
CAGCATTAGAGGTAACAACTAGAGTACAACAGCCACTAGTAGCCTATGCT226090.11958136525752228No Hit
CCAGCATTAGAGGTAACAACTAGAGTACAACAGCCACTAGTAGCCTATGC219670.11618576012260566No Hit
TCCAGCATTAGAGGTAACAACTAGAGTACAACAGCCACTAGTAGCCTATG205850.1088762176047634No Hit
CCCGACTGTCCCTGTTAATCATTACTCCGATCCCGAAGGCCAACACAATA202550.10713081309616142No Hit
CGGCAATCGGGCGGCGGGCGCATGCGTCGCTTCTAGCCCGGATTCTGACT196840.10411073438582283No Hit
CGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAG192910.102032116289215No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA190970.1010060300023399No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTCT294100.046.3272633
TACTCTA300700.045.3687484
TCTAGTT332350.040.9092867
TTGTACT336550.039.9230041
TGTACTC348800.039.0522352
ACTCTAG411500.035.8149955
CTCTAGT423000.033.6750456
TAGTTGT418000.032.4101879
ACCTACT306450.030.88725333
TGTTACC438350.030.72943913
GTTACCT440750.030.54622714
ACACCTA314000.030.1760331
TACCTCT449250.029.98362216
CTAATGC456700.029.23659921
CTAGTTG467300.029.0731498
TCTAATG463900.028.90216620
TCAGGAC312600.028.68361960
TGGACAC450550.028.61186428
ATGCTGG461900.028.56753324
TAATGCT469550.028.39199422
Produced by FastQC (version 0.11.5)