FastQCFastQC Report
Tue 27 Aug 2019
H3JVYBGXC_n01_0730-2R-CCA1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3JVYBGXC_n01_0730-2R-CCA1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25466938
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG326720.12829182683838944No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG276320.10850146177761928No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT270180.10609049270077148No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCAG97100.021.0485088
TACTCTA68900.019.9118884
TCTAGTT80200.018.9380937
CTGACTC109650.018.8315642
CGCTATG68500.018.701629
ACTCTAG202300.018.528495
GTCGCTA73250.017.48914327
GTACTCT81700.017.0491683
GTGCGCA153850.016.49229268
CTAATGC81050.016.40906521
CGCGGGG21150.016.221371
TGCGCAT160100.015.82660469
ACCTACT58500.015.79721933
ATGCTGG82450.015.70691124
CGTTCAG63750.015.700994557
TAGCCCG65450.015.56390234
GGCTGCC82950.015.52936743
GTTACCT87300.015.39366814
GAGGCGT67550.015.38714253
CTGTAGG60200.015.29071949
Produced by FastQC (version 0.11.5)