FastQCFastQC Report
Tue 27 Aug 2019
H3JVYBGXC_n01_0730-2G-SVP.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3JVYBGXC_n01_0730-2G-SVP.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26345641
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCTA90900.022.8316844
GTACTCT101050.020.3651353
TCTAGTT112350.019.7798527
ACTCTAG189650.019.1000565
ACCTACT79700.018.007533
CTAATGC113600.017.43846721
GTTACCT121250.016.65426814
GACTATC87700.016.40131455
ACACCTA88750.016.36705431
ATGCTGG128800.015.35394924
TACCTCT131550.015.323359516
CCTACTA94200.015.31002334
TGGACAC127150.015.14013328
TGTTACC137750.014.88800913
CTAGCAG80400.014.7563318
CTCTAGT171950.014.61412056
CGCTATG60900.014.59816129
CGCGGGG20200.014.557761
CTAGTTG142850.014.5036138
CTATCTA102700.014.41869238
Produced by FastQC (version 0.11.5)