FastQCFastQC Report
Tue 27 Aug 2019
H3JVYBGXC_n01_0730-1R-EV.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3JVYBGXC_n01_0730-1R-EV.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26643515
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCTA76400.020.522634
GACTGTC109750.018.2087424
TCTAGTT91500.018.1290347
ACTCTAG193100.017.4177135
ACCTACT67150.016.83825333
CTGTAGG68000.016.41926849
CTAATGC89500.016.26809321
GTACTCT95250.016.2406433
CTAGCAG94150.015.9091648
ATGCTGG92350.015.76648924
CGCTATG68800.015.71991629
CGCGGGG22400.015.6279311
GTTACCT96050.015.484920514
GTGCGCA125100.015.47051568
ACACCTA74000.015.37242631
GACTATC71750.015.31600655
CCTACTA76200.014.93039534
GTCGCTA74000.014.66303927
CTGACTC103450.014.5814552
TGCGCAT132250.014.55472569
Produced by FastQC (version 0.11.5)