FastQCFastQC Report
Tue 27 Aug 2019
H3JVYBGXC_n01_0730-12R-FBH3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3JVYBGXC_n01_0730-12R-FBH3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22711883
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT258550.11383908590934534No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCTA100800.027.8803374
GTACTCT105750.026.0787033
TCTAGTT115950.024.5378257
ACCTACT90200.021.81116933
CTAATGC128300.020.70514121
ACACCTA97300.020.54150631
GTTACCT138200.019.62492614
ATGCTGG137100.019.30015824
GACTATC98800.019.2695555
TAGTTGT146700.019.1561579
TACCTCT143200.019.06205616
CTGTAGG104900.018.48418249
ACTCTAG197400.018.4033575
TGTTACC149850.018.33277513
TGGACAC142100.018.30153328
TCTAATG147500.018.24636720
TAATGCT151600.017.7076622
TATCAGG111150.017.4436258
CCTACTA115750.017.42029234
TCAGGAC110250.017.36369560
Produced by FastQC (version 0.11.5)