FastQCFastQC Report
Tue 27 Aug 2019
H3JVYBGXC_n01_0730-12G-ABF1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3JVYBGXC_n01_0730-12G-ABF1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19674489
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT495950.2520777032633478No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA404310.20549961932937624No Hit
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATG246130.125101089029555No Hit
CAGCATTAGAGGTAACAACTAGAGTACAACAGCCACTAGTAGCCTATGCT198000.10063793778837153No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCTA185750.039.8139044
GTACTCT197050.037.246083
ACTCTAG240250.036.8571975
TCTAGTT209750.035.239047
CTCTAGT220850.033.533856
TGTACTC230750.031.9583782
TTGTACT231600.031.3805891
CTAGTTG233800.031.3000458
TATGCCG33650.028.70782346
GCACACG37350.027.6414811
ACCTCTA269000.027.0476717
GTTACCT268950.026.98763814
CTAATGC268400.026.96655721
CGTATGC35950.026.87320944
TACCTCT273700.026.45540816
ACCTACT149450.026.3974333
GTATGCC35850.026.36173845
TGTTACC276350.026.3283613
ATGCTGG277550.025.73792824
TAGTTGT292350.025.6300249
Produced by FastQC (version 0.11.5)