FastQCFastQC Report
Tue 27 Aug 2019
H3JVYBGXC_n01_0730-11R-HSFA6A.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3JVYBGXC_n01_0730-11R-HSFA6A.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22376444
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG246530.1101738953696128No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG230940.10320674723830114No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCTA61050.019.8925274
CTAGCAG78200.019.5572688
ACTCTAG161350.018.5457025
TCTAGTT74400.016.416237
GTACTCT70850.016.3505123
CTGACTC94800.015.7639732
GACTGTC84350.015.4349064
CGCTATG53800.015.28852929
CAGGGGA58700.015.0882621
CTAATGC74750.015.02984621
ACCTACT50800.015.02245233
CGCGGGG18650.014.8287671
GTGCGCA117450.014.720283568
ATGCTGG76600.014.21038624
GTGTGCG55850.013.91115958
TCTAGCA190850.013.7894387
TGCGCAT126550.013.74473969
CGGCAAT65450.013.5856641
CTGTAGG55700.013.50940549
GACTCTA140250.013.4752554
Produced by FastQC (version 0.11.5)