FastQCFastQC Report
Tue 27 Aug 2019
H3JVYBGXC_n01_0730-11G-ZAT6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3JVYBGXC_n01_0730-11G-ZAT6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20200723
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT479730.23748159904969737No Hit
CTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGAC213110.10549622407079193No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA212010.104951689105385No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCTA156200.036.8802384
GTACTCT160600.035.7171863
TCTAGTT182300.031.7516927
ACCTACT141250.026.74081433
TGTACTC222000.025.7916722
ACTCTAG255550.025.5553445
GTATGCC24750.025.4572245
ACACCTA147450.025.4475831
CTAATGC223350.025.05680521
TTGTACT225300.024.9851911
GTTACCT227900.024.96909714
CGTATGC25100.024.82406244
TAGTTGT237700.024.5730559
TGTTACC233800.024.42880613
TACCTCT233500.024.416
TGGACAC223500.024.3832428
ATGCTGG229450.024.25427624
GCACACG28600.023.49449311
TCTAATG240250.023.42400220
CTCTAGT261350.023.3010736
Produced by FastQC (version 0.11.5)