FastQCFastQC Report
Tue 27 Aug 2019
H3JVYBGXC_n01_0730-10R-CCA1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3JVYBGXC_n01_0730-10R-CCA1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39909196
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG544290.13638210100749712No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG442880.11097191734957526No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC422330.10582272817523058No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCTA145550.025.9694774
TCTAGTT162950.024.1186877
GTACTCT165900.022.5940253
ACTCTAG346500.020.9790845
ACCTACT130150.020.95202633
CTAGCAG158700.019.759098
ACACCTA141450.019.62307431
CTAATGC188750.019.3586221
GTTACCT197800.018.79003314
ATGCTGG199300.018.21148724
TGGACAC196600.018.05219528
CTGTAGG151500.018.01927849
TAGTTGT215950.017.956539
CTGACTC175350.017.8437732
GACTATC150000.017.80212455
TACCTCT210350.017.7020516
CCTACTA158200.017.36988634
TCTAATG217050.016.9143320
TGTTACC222250.016.91187713
TCAGGAC156450.016.6656460
Produced by FastQC (version 0.11.5)