FastQCFastQC Report
Tue 27 Aug 2019
H3JVYBGXC_n01_0730-10G-SVP.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3JVYBGXC_n01_0730-10G-SVP.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34374611
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT552190.16063890875739656No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCTA169150.033.8708654
GTACTCT181150.031.2017333
TCTAGTT199350.028.68567
ACCTACT174150.024.48305733
ACTCTAG328850.023.2437215
ACACCTA183950.023.1577331
CTAATGC240000.022.95389221
GTTACCT241600.022.94467714
TACCTCT253100.022.01263816
TGGACAC244350.021.78722228
GACTATC192450.021.58569155
ATGCTGG253600.021.37883224
TGTTACC261150.021.32078613
TCTAATG266000.020.70925320
CTGGACA260600.020.68426727
TCAGGAC198550.020.57032260
TAATGCT268950.020.5353222
CAGGACT199900.020.36134161
CTCTAGT306850.020.199836
TATCAGG208250.020.04898658
Produced by FastQC (version 0.11.5)