FastQCFastQC Report
Thu 27 Feb 2020
H3JTHAFX2_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3JTHAFX2_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8225140
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5963777.250660779026254No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGATC7500.024.222342
GGTCGAC9550.020.1360661
GCGATCT8950.018.7270813
GGGCGAT10100.018.6933731
TATCGCG2056.675E-717.07504822
GTCGACC11700.015.5271412
TGGCGCG31450.015.3218193
CGCGTGC29700.015.1514616
CGATCTG11200.014.9584814
CGTGCCT30400.014.3421028
GCGTGCC32200.014.2988037
GGCGCGT32700.014.1960714
TCGACCC12850.013.8585863
GCGACAT12400.013.50768114
ACCCGTG13500.013.4366456
GTAGTCC30950.013.30538615
TGTAGTC32250.013.09232814
TAGTCCC30900.012.76244816
GCGCGTG37600.012.5269045
GACCCGT14500.012.5121855
Produced by FastQC (version 0.11.5)