FastQCFastQC Report
Thu 27 Feb 2020
H3JTHAFX2_n02_WT_04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3JTHAFX2_n02_WT_04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24948506
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1843090.7387576634849398No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGA260110.10425874799877795No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGAT46950.025.624451
GGCGATC48250.024.5550962
TGGCGCG190950.020.421383
GGTCGAC86700.020.3086341
AGGTCGA72450.020.2437766
GGTGGCG201000.019.9979111
GGCGCGT210400.018.459074
GCGATCT65200.017.9476853
GTGGCGC221950.017.751432
TATCGCG6550.017.09769422
CGATCTG73900.016.1597254
TGCGACA70350.015.86959613
TCGACCC116600.015.2642243
GTCGACC115600.015.161232
GCGTGCC258500.015.05627257
CGACCCG126600.015.0206994
GCCACGA64450.014.87864919
CGCGTGC264300.014.8597536
CGTGCCT261900.014.8203338
GCGACAT76550.014.81283714
Produced by FastQC (version 0.11.5)