FastQCFastQC Report
Thu 27 Feb 2020
H3JTHAFX2_n02_WT_03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3JTHAFX2_n02_WT_03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13736148
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2090851.5221516250407319No Hit
GTTACAGCCCCCCCGGCAGCAGCACTCGCCGAATCCCGGGGCCGAGGGAG142750.10392287561258078No Hit
GCAGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGACCCGTCGCCGCGC140550.1023212621180261No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCG9800.046.78129622
ATATCGC15700.030.53851721
GGCGATC25500.028.454242
GGGCGAT29400.025.0533581
TGGCGCG84750.024.804053
TAACACG26100.023.4818844
ATTCGCG6950.023.16319728
GGCGCGT91750.022.7496954
GTCTAAC28500.022.6447221
GCGATCT34400.021.591783
CGCGATT7950.021.13015731
TCGCGAT7750.020.77208130
TTCGCGA8300.020.66062429
GATATCG23600.020.46431720
CGCGTGC109100.019.2182066
ACGTGCG32550.018.8168668
TTACAGC73100.018.5091632
TCGCACG32800.018.35185827
GGTGGCG117900.018.2662161
TCGCGGC26600.018.15611524
Produced by FastQC (version 0.11.5)