FastQCFastQC Report
Thu 27 Feb 2020
H3JTHAFX2_n02_WT_02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3JTHAFX2_n02_WT_02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27683590
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1732630.6258689714737142No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCG13300.039.207122
GGCGATC50250.026.5077692
GGGCGAT54750.024.6658551
ATATCGC22500.023.33129321
GCGATCT66050.020.1036663
GGCGCGT164450.019.527744
TGGCGCG170800.019.0562173
GGTCGAC74050.018.8528751
CGACGTA11150.018.51832635
TCGTATA14700.018.0934341
CGTATAA16100.016.52008642
CGATCTG84100.016.489814
CGCGTGC196650.016.3048616
GTGGCGC202300.016.1316742
GGTGGCG204000.016.111241
GCGCGTG205250.015.8962495
TGCGACA80300.015.8645213
CGCTAAT12950.015.67410834
AGGTCGA72650.015.6589756
GCGACAT89400.014.79773914
Produced by FastQC (version 0.11.5)