FastQCFastQC Report
Thu 27 Feb 2020
H3JTHAFX2_n02_WT_01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3JTHAFX2_n02_WT_01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10210390
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1390501.3618480782810451No Hit
GGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGAC234190.22936440233918587No Hit
GCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGC231270.2265045703445216No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGA180980.1772508199980608No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGG167130.16368620591378No Hit
GTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACC165320.16191350183489564No Hit
GGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAAC152150.14901487602334484No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGA136200.1333935334497507No Hit
GTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTG119630.11716496627454974No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCG5250.049.9952922
ATATCGC8850.030.8445521
AGGTCGA60250.029.9782966
GGGCGAT23900.029.7907331
GGCGATC24150.028.5929832
TGGCGCG96800.027.3994183
CGACGTA4800.026.97662435
GGTGGCG101950.026.5590881
GGCGCGT105200.025.3330524
GCGATCT28250.024.6805823
GTGGCGC110400.024.1616132
GGTCGAC83550.023.130671
TGCAGGT82100.022.5326143
CGCTAAT5050.022.1760234
TCGTATA5350.020.93250541
CGATCTG32850.020.8948524
GCGTGCC128700.020.614217
CGCGTGC128550.020.5583936
CGTATAA4950.020.50301742
CGTGCCT127850.020.4772998
Produced by FastQC (version 0.11.5)