FastQCFastQC Report
Thu 27 Feb 2020
H3JTHAFX2_n02_N3_04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3JTHAFX2_n02_N3_04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15924454
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG559590.35140294292036633No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGA278630.1749698922173407No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGA229320.14400493731213643No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGG207620.1303780964797914No Hit
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGAT190560.11966501331851001No Hit
GGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATT176510.1108421048533281No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGG173070.108681905200643No Hit
GTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTG164360.10321232991724551No Hit
GGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCG162410.1019877981373804No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGATC42650.034.438142
GGGCGAT45450.032.8811531
GCGATCT54400.026.8567753
GGTGGCG182700.023.2720871
CGATCTG66900.022.1431034
CGGTGGC130950.021.7263431
TGGCGCG198750.021.012893
GTGGCGC216450.019.466672
GGCGCGT214850.019.2992574
GGTCGAC35400.017.6395741
GCGCGTG242600.017.172485
TGCGACA84600.017.04383713
CGCGTGC250700.016.5876876
GCGACAT89350.016.25521314
TATCGCG4100.015.36444522
GCGTGCC279400.015.0077157
GCTGCGA97400.014.87613411
CGTGCCT281400.014.7513738
CTGCGAC98600.014.58832412
CCTGTAG302050.013.89251312
Produced by FastQC (version 0.11.5)