FastQCFastQC Report
Thu 27 Feb 2020
H3JTHAFX2_n02_N3_03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3JTHAFX2_n02_N3_03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20880237
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1671710.8006183071581037No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT310160.1485423752613536No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGA265470.1271393614928796No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGG250710.12007047621154873No Hit
GGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAAC247750.11865286778114635No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGATC48400.031.8669822
CGGACAC86150.028.76216113
ACGGACA90450.027.66539418
TCACCCG92800.027.145313
ACACGGA92200.027.10233316
CACGGAC94400.026.47077417
CTCGATT94850.026.12286249
CGGACAG96700.025.87711319
TCTCGAT96050.025.72362348
GCGATCT60650.025.4754353
TTCTCGA97550.025.36395347
CCGGACA97700.025.32610312
ATTCCGT96700.025.15273553
TTTCTCG99000.024.99246246
GACACGG102350.024.72241215
TAGCATA56200.024.3502089
ATAGCTC103350.023.97439239
GGACACG108950.023.32119814
TGATAGC106450.023.01326437
CGATCTG70900.022.2766324
Produced by FastQC (version 0.11.5)