FastQCFastQC Report
Thu 27 Feb 2020
H3JTHAFX2_n02_N3_02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3JTHAFX2_n02_N3_02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43761895
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3765090.8603580809286252No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGA548120.12525051760212852No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGG523640.11965660993428187No Hit
GGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAAC454890.10394659554847888No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGA441180.10081373304332455No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGATC90800.027.9500642
GGGCGAT94400.027.2731021
TATCGCG13550.027.1192622
GCGATCT119150.021.8768793
TGGCGCG364150.021.6186833
GGTGGCG381750.021.1880381
GGCGCGT371700.021.0856134
GTGGCGC421650.018.904642
CGATCTG140400.018.4080584
CGCGTGC430100.018.2464716
GGTCGAC87050.017.971181
GCGCGTG443500.017.7689255
TGCGACA150700.016.4886613
GCGTGCC489300.016.0947517
CGTGCCT487600.016.0931118
GCGACAT163650.015.44050314
GTAGTCC510050.015.34945715
TAGTCCC513250.015.24010116
AGGTCGA92350.015.1991826
CCTGTAG527100.014.98579412
Produced by FastQC (version 0.11.5)