FastQCFastQC Report
Thu 27 Feb 2020
H3JTHAFX2_n02_N3_01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3JTHAFX2_n02_N3_01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16012237
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1944771.2145523451844986No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGA309160.19307733204298688No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGA240180.14999777982301912No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGG237970.14861758541295636No Hit
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGAT217750.1359897433444184No Hit
GGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAAC197960.12363044588960306No Hit
GTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTG187130.11686686875793807No Hit
GGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATT169310.1057378803473868No Hit
GGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCG164760.10289630362078701No Hit
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGAT160170.10002974599988745No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGAT42600.029.804171
GGCGATC42000.029.7954312
GGTGGCG174900.025.7686331
GCGATCT51350.024.9723593
TGGCGCG184400.024.3012473
GGCGCGT197750.022.544514
GTGGCGC203850.022.2512452
CGATCTG58050.021.5978094
CGCGTGC222850.019.979396
GCGCGTG227450.019.63975
TATCGCG4350.019.30861322
GGTCGAC32950.018.5213181
CGTGCCT240550.018.3763648
GCGTGCC245900.018.218867
CCTGTAG256400.017.36258712
AGGTCGA34500.017.1464946
GTAGTCC256850.017.00514615
TAGTCCC257200.016.96839716
TGCGACA73450.016.96303413
TGTAGTC261000.016.85544214
Produced by FastQC (version 0.11.5)