FastQCFastQC Report
Thu 27 Feb 2020
H3JTHAFX2_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3JTHAFX2_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8225140
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1955812.3778440245394No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG111100.13507368871532885No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG13650.025.58718315
CGTCTGA13800.025.05566816
ACACGTC13750.024.13427213
CACGTCT14900.022.502314
CCGATCG38050.021.77608770
ACCCGAT36400.021.22539968
CACACGT16500.020.534312
CCCGATC40850.020.19678769
ACCGCGT7550.018.4898196
GCACACG21100.017.8679411
CACCCGA45800.017.63798967
ATACGGA7500.015.90166933
AATACGG7750.015.85634132
TATCGCG2653.5912308E-815.8172222
GTCGGTT7850.015.5656021
TGTATCG3401.8371793E-1015.51725237
TAGTGCG59750.015.09648558
ACACCCG52600.015.02509866
ATCGGAA26850.014.817862
GATCGGA25450.014.4035331
Produced by FastQC (version 0.11.5)