FastQCFastQC Report
Thu 27 Feb 2020
H3JTHAFX2_n01_WT_04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3JTHAFX2_n01_WT_04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24948506
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG907360.3636931205419675No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG463090.18561832920977311No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG377700.1513918308374858No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT374710.1501933622798896No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT355840.14262978312208355No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA333250.1335751327153618No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT315650.1265206020753307No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC254970.10219850439140525No Hit
GGGAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCT250070.10023445892912385No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTACG38050.040.5720672
CGTACGG38900.039.6850553
GGCGTAC53200.030.93671
GTACGGA59150.026.690694
ACCCGAT251950.026.37967368
CCCGATC255500.026.34190869
ACGGTCG37350.025.95666154
TACGGAA61000.025.8238325
ACCGCGT39450.024.13576
TATGCCG17550.024.13058946
CCGATCG302650.023.63736370
AGACCGC41400.023.5062034
GATCGTT55900.023.4205885
CGGTCGA40650.023.33288455
TGATCGT57600.022.9724524
TCGTATG17650.022.80409243
ATACGGA43250.022.2538633
AATACGG45350.021.53206432
CGCCAAT33550.021.28121633
CACCCGA316300.021.2009467