FastQCFastQC Report
Thu 27 Feb 2020
H3JTHAFX2_n01_WT_03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3JTHAFX2_n01_WT_03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13736148
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG508420.3701328785915819No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG199640.14533914456949648No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG161420.11751475013227872No Hit
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT139930.10186989831501524No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCG11800.037.96511522
CGCGATT5500.033.0900531
ATATCGC15100.029.89988521
ATTCGCG6300.028.88813828
CGCTAAT7000.028.4992634
ATCGCGG16250.027.56851223
CCGATCG54400.027.27870270
TTCGCGA6700.027.16347329
ACCCGAT57450.025.4649668
TCGCGAT6900.024.85442730
GATATCG19100.023.45488720
CCCGATC66550.022.77175969
CCGCTAA9250.021.18863733
CACCCGA71150.020.6108567
GTCGACA12900.018.4599511
ACCGCGT18550.018.116766
CGACAAC10950.017.9030533
GTCGGAT11750.017.8823551
TAGTGCG85850.017.3670558
ACACCCG84300.017.14664866
Produced by FastQC (version 0.11.5)