FastQCFastQC Report
Thu 27 Feb 2020
H3JTHAFX2_n01_WT_02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3JTHAFX2_n01_WT_02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27683590
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG493890.17840532965558298No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG481860.17405979499046184No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT307450.11105857296687316No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG304760.11008687818306802No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT287470.10384130092953985No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT280580.10135246187362261No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCG14450.042.62872322
ATATCGC24200.025.30930121
CCGATCG150850.023.01568270
ATCGCGG27250.021.96276923
ACCGCGT44550.020.508966
ACCCGAT169000.020.4610468
CCCGATC180850.019.6235269
AATACGG49200.018.851232
AGACCGC49300.018.674964
TCGACGT14700.018.57097834
GTCGGTT41800.018.2635351
GAACGAT52000.017.97074945
AACGATC52450.017.54965246
GATATCG34600.017.49957520
ATACGGA52350.017.44943233
CACCCGA202150.017.34808267
AAGAACG53800.017.2393943
GACCGCG55000.016.8668525
CAGACCG59700.016.653093
TACGGAG55300.016.58187734
Produced by FastQC (version 0.11.5)