FastQCFastQC Report
Thu 27 Feb 2020
H3JTHAFX2_n01_WT_01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3JTHAFX2_n01_WT_01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10210390
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG342160.335109628525453No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG288760.282809961225771No Hit
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT225190.22054985167070015No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG217830.21334150801291624No Hit
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT204960.20073670055698167No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA142880.13993588883480454No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA137950.1351074738575118No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT135910.1331095090393217No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA135710.13291363013557758No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT124370.12180729629328556No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG117990.11555875926384791No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT115060.11268913332399644No Hit
GTCCGCTCCGGGCCGGGACGGGGTCCGGGGAGCGTGGTTTGGGAGCCGCG109630.10737102108734338No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC103630.10149465397501956No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTATCG33900.060.39697337
ACCGATG55800.056.70105432
GTATCGG23750.055.4093738
CACCGAT58500.054.26329431
GTATCGC13750.052.6898138
CCGATGT61700.051.16562333
ATCGCGT7700.050.9080340
GATGTAT59850.050.23262835
TCACCGA64350.049.656630
CGATGTA63900.049.56836734
ATGTATC57700.048.46496236
CGTCTGA68900.046.63160316
GTCACCG68850.046.3602429
ACACGTC75650.042.47081813
ACGTCTG77800.041.56705515
CACGTCT78800.040.9951414
CACACGT83550.038.49691812
AGTCACC84750.038.15812328
TATCGCG18050.038.0047539
CGCGTAT6300.036.66590542
Produced by FastQC (version 0.11.5)