FastQCFastQC Report
Thu 27 Feb 2020
H3JTHAFX2_n01_N3_03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3JTHAFX2_n01_N3_03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20880237
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG786590.37671507272642546No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG409440.19608972829187715No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT407450.19513667397549175No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG378250.18115215837827894No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA356270.1706254579390071No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT350720.1679674421320026No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT292710.14018519042671787No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG292560.14011335216166368No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT280460.13431839878062685No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT254920.12208673685073594TruSeq Adapter, Index 13 (97% over 40bp)
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC233630.11189049243071332No Hit
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT218740.10475934731966884No Hit
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT218340.1045677786128577No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG37400.052.77942745
TATGCCG37650.051.40641848
CGTCTGA72600.038.27727516
GTATGCC53450.036.93078647
ACACGTC85350.032.76426313
ACGTCTG85250.032.76164215
ATGCCGT56850.032.5673549
AGTCAAC89950.031.16653434
CACGTCT91350.030.76551414
CGTATGC67600.029.92528546
CACACGT100750.028.06877712
GCCGTCT58350.026.99161151
TGCCGTC69350.025.94023150
CGTGCGA54850.025.45970738
GCGATCG59450.023.54861341
CGTACGG11850.023.3383943
CCGATCG259650.023.29231370
ACCGCGT27000.022.949426
ACCCGAT266350.022.25962668
GCACACG130300.022.24042511
Produced by FastQC (version 0.11.5)