FastQCFastQC Report
Thu 27 Feb 2020
H3JTHAFX2_n01_N3_02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3JTHAFX2_n01_N3_02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43761895
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG1340950.30641954604570937No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG872610.19939950041011706No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG815600.18637218520815882No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA677790.15488131855350412No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT589370.13467652623361032No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT545200.12458327044566969No Hit
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT540060.123408732642862No Hit
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT510210.11658773003317155No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT495350.11319208183283654No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA471080.10764616111802287No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG438100.10010992439884059No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA120400.029.9119816
TCGTATG36350.029.5591743
TATGCCG37650.027.60884546
ACGTCTG135700.026.82314915
ACACGTC136400.026.60851713
CCGATCG413500.024.06343570
CACGTCT152100.024.00000814
ACCCGAT442100.022.1663368
ACCGCGT77200.021.7211426
CCCGATC461200.021.6429569
CACACGT172500.021.18203412
AATACGG79950.020.39972132
AGACCGC82350.020.2352144
GTCGTAT30100.020.23210142
ATACGGA83850.019.70131533
CACCCGA510850.019.31336467
TACGGAG87850.018.9237934
GTCGGTT76550.018.664781
GACCGCG93750.017.9986635
GAACGAT92600.017.95309645
Produced by FastQC (version 0.11.5)