FastQCFastQC Report
Thu 27 Feb 2020
H3JTHAFX2_n01_N3_01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3JTHAFX2_n01_N3_01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16012237
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG768300.47982052726299274No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG513620.3207671732563039No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG379470.23698749899842228No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA377430.23571347338913357No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT290650.18151742320576444No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT273660.1709067883519336No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT248330.15508763703659895No Hit
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT247550.15460050959775326No Hit
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT238100.14869877331943065No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT230950.14423343846334524No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG226070.1411857693587723No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA224990.14051128521267828No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA199930.12486075493386714No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC198850.12418627078777313No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC174760.10914152719573161No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG173190.10816102709446532No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCG211000.026.4401670
ACCCGAT217800.025.10044568
CCCGATC225800.024.7071569
CACCCGA252950.021.75084567
CGTCTGA31900.021.72364816
ACCGCGT22950.019.981636
GTCGGTT27100.019.8999121
AATACGG23850.019.81087532
ACACGTC35850.019.5253613
ACGTCTG37450.018.59771215
TGGGGGG211450.018.4491861
AGACCGC25850.018.1462384
TACGGAG25700.018.11243234
ACACCCG303200.017.86898866
ATACGGA26350.017.66563633
CGTACGG8150.017.6103743
CGCGTTC27850.017.2168468
TAGTGCG334300.017.00230858
GTCCGTT14950.016.8651811
GTCCGAT17500.016.8089621
Produced by FastQC (version 0.11.5)