FastQCFastQC Report
Tue 29 May 2018
H3HF7AFXY_n02_wt-asynch.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3HF7AFXY_n02_wt-asynch.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12687540
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8136276.412803427614809No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC3081022.4283824917990406Illumina Single End PCR Primer 1 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGA234900.062.9874319
ACACGAG235600.062.784220
CACGAGA248050.060.154721
CACGTAG178500.059.322921
GAGCACA756600.059.236216
AGAGCAC774400.059.2357515
ACACGTA178450.058.04549420
GCACAAG228450.057.50923518
AAGAGCA972950.057.3717614
GCACACG473000.056.1732518
AGCACAC534250.056.10225717
GCAGATC283500.054.4838875
AGCACAA246800.053.34845417
ACGAGAT279450.052.9691222
GTGACTG77250.051.395021
CCAGATC218750.051.38125
AGAGAGA301900.050.36232815
GAGCAAG171600.050.195916
CACAAGA263250.049.7303219
ACGTAGA213250.049.5897522
Produced by FastQC (version 0.11.2)