FastQCFastQC Report
Tue 29 May 2018
H3HF7AFXY_n02_wt-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3HF7AFXY_n02_wt-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16757720
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4666412.784632993032465No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC456700.2725311080504985Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGG52650.039.8924379
CGGGCTC45000.036.47861513
TACGGGC47850.034.8241811
GGGCTCG48700.033.99431614
TAGGGGG121900.033.1232938
GCACACG76450.032.45657318
CTCGAGT50150.032.45086717
GGCTCGA51200.032.3343415
CGGGGGA45700.032.16021349
AGAGCAC128600.032.14265415
GAGCACA126600.031.544316
CTTAGGG49600.031.40042961
ACACGAG41450.031.06826420
AGCGGGG74450.030.84545110
GCTCGAG54050.030.82337616
CACACGA42750.030.04262419
CCGGAGT54900.029.57475328
GTGACTG53950.029.4578441
AGCACAC97000.029.15304617
TTACGGG58500.028.66366210
Produced by FastQC (version 0.11.2)