Basic Statistics
Measure | Value |
---|---|
Filename | H3HF7AFXY_n02_triple-1.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 13266463 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 40 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC | 156174 | 1.177209027002902 | Illumina Single End PCR Primer 1 (100% over 50bp) |
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG | 136779 | 1.031013315304916 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGCCGTA | 30320 | 0.0 | 41.527233 | 47 |
GCCGTAT | 31380 | 0.0 | 40.78243 | 48 |
CCGTATC | 30725 | 0.0 | 40.638004 | 49 |
TCGCCGT | 30905 | 0.0 | 40.43513 | 46 |
GGTCGCC | 31465 | 0.0 | 40.21566 | 44 |
CGTATCA | 32195 | 0.0 | 39.11007 | 50 |
GTGGTCG | 32590 | 0.0 | 39.06262 | 42 |
GTCGCCG | 32915 | 0.0 | 38.635704 | 45 |
CTCGGTG | 32065 | 0.0 | 38.430458 | 38 |
TCTCGGT | 32705 | 0.0 | 37.592266 | 37 |
GGTGGTC | 34020 | 0.0 | 37.398674 | 41 |
ATCTCGG | 32970 | 0.0 | 37.387173 | 36 |
TCGGTGG | 34745 | 0.0 | 36.78701 | 39 |
GTATCAT | 34110 | 0.0 | 36.719143 | 51 |
GATCTCG | 33365 | 0.0 | 36.673565 | 35 |
AAAGGGG | 21265 | 0.0 | 36.060535 | 66 |
CGGTGGT | 35510 | 0.0 | 35.94767 | 40 |
AGATCTC | 35400 | 0.0 | 34.911564 | 34 |
TGGTCGC | 36475 | 0.0 | 34.76796 | 43 |
AGTGTAG | 38065 | 0.0 | 34.198986 | 29 |