FastQCFastQC Report
Tue 29 May 2018
H3HF7AFXY_n02_triple-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3HF7AFXY_n02_triple-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13266463
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1561741.177209027002902Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1367791.031013315304916No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA303200.041.52723347
GCCGTAT313800.040.7824348
CCGTATC307250.040.63800449
TCGCCGT309050.040.4351346
GGTCGCC314650.040.2156644
CGTATCA321950.039.1100750
GTGGTCG325900.039.0626242
GTCGCCG329150.038.63570445
CTCGGTG320650.038.43045838
TCTCGGT327050.037.59226637
GGTGGTC340200.037.39867441
ATCTCGG329700.037.38717336
TCGGTGG347450.036.7870139
GTATCAT341100.036.71914351
GATCTCG333650.036.67356535
AAAGGGG212650.036.06053566
CGGTGGT355100.035.9476740
AGATCTC354000.034.91156434
TGGTCGC364750.034.7679643
AGTGTAG380650.034.19898629