FastQCFastQC Report
Tue 29 May 2018
H3HF7AFXY_n02_rad27-exoi-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3HF7AFXY_n02_rad27-exoi-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13320070
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3939572.9576195920892308No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC583330.43793313398503164Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGAG44350.041.58167620
CACACGA44350.041.26746419
GAGCACA146600.041.13653616
AGAGCAC151600.040.86582615
CACGAGA50000.036.8825321
GCACACG90900.036.65251518
AGCACAC109100.033.77879317
CACGTAG39700.033.40633421
CAGATCG173800.033.3486186
GCACAAG67600.032.6155418
CGGGCTC30950.032.23196813
AAGAGCA261050.032.03196714
GGGCTCG31850.031.5401414
TACGGGC32250.031.15409711
AGAGATC138300.030.11378317
CTCGAGT34850.029.42424617
CTTAGGG31500.029.22113861
GAGAGAT143450.029.0594716
GCAGATC75500.028.3462585
GCTCGAG35650.028.27544216
Produced by FastQC (version 0.11.2)