FastQCFastQC Report
Tue 29 May 2018
H3HF7AFXY_n02_rad27-dna2-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3HF7AFXY_n02_rad27-dna2-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11909093
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2676422.2473751779417626No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC887960.7456151362660448Illumina Single End PCR Primer 1 (100% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGA56500.049.11909519
ACACGAG57550.048.40249620
CACGAGA63100.044.36612721
AGAGCAC194600.042.86165215
GAGCACA192100.042.1613516
CACGTAG49850.040.22354521
GTGACTG51650.039.046421
GCACACG132800.039.03053318
AGCACAC151750.037.70874417
TGACTGG56950.036.8622632
CGGGCTC37700.036.76605213
GACTGGA60650.035.8142743
GGGCTCG39350.035.491414
TACGGGC39550.035.31608611
CTCGAGT41100.034.65816517
GCGGCGC19900.034.545121
AAGAGCA301500.034.23595414
GCTCGAG41550.034.03211216
GCACAAG64800.033.91814818
ACGAGAT82750.033.7038422
Produced by FastQC (version 0.11.2)