FastQCFastQC Report
Tue 29 May 2018
H3HF7AFXY_n02_rad27-asynch.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3HF7AFXY_n02_rad27-asynch.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11127878
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7026506.314321562475793No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1809911.6264646323405056Illumina Single End PCR Primer 1 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGAG191400.062.39938420
CACACGA191900.062.16637819
AGAGCAC603000.059.65725315
GAGCACA588950.059.65308816
CACGAGA203450.059.56384321
CACGTAG150650.059.3484921
GCACACG382300.057.83576218
ACACGTA149800.057.69937520
AGCACAC430050.057.03874617
AAGAGCA750000.056.68651214
GCACAAG170550.055.56714618
GCAGATC222250.053.297025
ACGAGAT224500.053.05877722
ACGTAGA175950.050.77448722
CCAGATC161500.050.4221735
AGCACAA191700.049.4013217
CAGATCG678500.049.279166
CACACGT202550.048.5659819
GAGAGAT228050.046.87087616
CGTAGAT198700.046.73940323
Produced by FastQC (version 0.11.2)