FastQCFastQC Report
Tue 29 May 2018
H3HF7AFXY_n02_rad27-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3HF7AFXY_n02_rad27-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15264799
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4773023.1268148371950395No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC489160.32044968296012283Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGCGC27300.037.5168881
GAGCGGG43900.036.280519
ACACGAG34800.035.79908420
AGAGCAC116450.034.7465815
GAGCACA114550.034.4358816
CACACGA37250.033.63339219
TAGGGGG100200.032.19513738
CGGGGGA41350.031.65098649
GCACACG66950.031.31277318
CACGAGA41400.031.10621621
AGCGGGG60100.028.59889210
AGCACAC86950.027.2504917
CAGATCG135400.026.30316
CGGCGCT38550.026.1848012
TAGATCG142350.025.3387056
GGAGAGC51300.024.0969456
CGGCGTT23950.023.7255381
CACGTAG32650.023.25820721
GCACAAG63750.022.6733218
AAGAGCA234600.022.21564914
Produced by FastQC (version 0.11.2)