FastQCFastQC Report
Tue 29 May 2018
H3HF7AFXY_n02_exoi-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3HF7AFXY_n02_exoi-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12514684
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5346834.272445073323466No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC183060.14627616646173408Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC82100.031.63220815
GAGCACA78950.031.51924716
GAGCGGG26400.030.4973589
CACACGA26550.029.2610219
TAGGGGG55550.028.28059838
GCACACG45050.028.27679618
ACACGAG27250.027.60964420
TAGATCG94650.026.6031236
CAGATCG91350.025.4939676
AGCACAC60300.024.4921817
CGGGGGA28350.024.44047749
CACGAGA31600.023.69773721
GATCGGA335950.022.1874378
AGATCGG337150.022.0691387
AGAGATC81550.022.01535417
TCGGAAG337950.021.72191810
AGCGGGG40650.021.4432710
CGGGGAC12100.020.870661
CGGAAGA350650.020.84500111
ATCGGAA356200.020.7360889
Produced by FastQC (version 0.11.2)