FastQCFastQC Report
Tue 29 May 2018
H3HF7AFXY_n02_dna2-exoi-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3HF7AFXY_n02_dna2-exoi-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15158613
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4688003.0926312321582454No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC383980.25330813577733Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC127700.032.1222515
GAGCACA125650.031.39232616
CAGATCG186900.030.689456
ACACGAG36850.030.38855220
CACACGA38000.029.74575619
AGAGATC141300.029.5240617
GTGACTG51000.029.2296141
GCACACG74950.028.43687418
TGACTGG55100.027.860052
TAGATCG210100.027.6672866
GAGAGAT153250.027.22311816
GACTGGA60350.026.8782353
CGGGCTC25050.026.40743313
AGCACAC94550.025.57752417
CACGAGA46500.024.9849421
CACGTAG34100.024.62918721
GGGCTCG28000.024.25006714
GCGGCGC18600.023.9494251
GCAGATC79850.023.8166795
TACGGGC27950.023.67131611
Produced by FastQC (version 0.11.2)