FastQCFastQC Report
Tue 29 May 2018
H3HF7AFXY_n02_dna2-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3HF7AFXY_n02_dna2-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12230949
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3977593.2520698107726558No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC216410.1769363930795558Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG31400.031.20834518
AGAGCAC65650.030.44367415
GAGCACA66450.028.91701716
GAGCGGG27050.027.9521969
TAGGGGG55700.026.82273138
CACACGA23000.024.49724619
ACACGAG22950.024.39772820
AGCACAC48950.023.165617
CGGGGGA24600.022.61866849
CAGATCG70050.022.4438576
TAGATCG73400.021.848866
AGCGGGG41500.020.75043110
CACGAGA27850.020.73307621
CGGGGTT18300.020.1226331
GATCGGA267600.019.2966378
AGATCGG269900.019.003897
TCGGAAG274500.018.75903510
CGCCGTA104250.017.99281347
AAGAGCA158100.017.8885514
GGGAGAG103850.017.82564523
Produced by FastQC (version 0.11.2)