FastQCFastQC Report
Tue 29 May 2018
H3HF7AFXY_n01_wt-asynch.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3HF7AFXY_n01_wt-asynch.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12687540
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTCGTATG2876542.2672164974455256TruSeq Adapter, Index 3 (97% over 37bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG131660.1037711014113059No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGG89350.045.24498770
ACGTGTG360250.040.6282651
CTTGAAA1532850.039.5011367
TGAAAAA1590450.039.0692269
GAAAAAA1614650.038.8500170
GCTTGAA1553050.038.76018566
TTGAAAA1585150.038.40289368
TGCTTGA1561550.038.1319865
AAAAAGG449650.038.1265370
CTGCTTG1570350.037.92260764
TCTGCTT1628750.036.6349263
CTTCTGC1661850.036.38079561
TTCTGCT1646100.036.15919562
GTCTTCT1733400.034.89148359
TCTTCTG1729350.034.88239760
AAAAAAG542050.032.22784869
CCGTCTT1919800.031.80506757
CGTCTTC1918800.031.7901358
GCCGTCT2016100.030.2736156
CGTGTGC621600.029.7425981
Produced by FastQC (version 0.11.2)