FastQCFastQC Report
Tue 29 May 2018
H3HF7AFXY_n01_wt-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3HF7AFXY_n01_wt-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16757720
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC5590723.33620564134023TruSeq Adapter, Index 1 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG414190.24716369529983792TruSeq Adapter, Index 1 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGAGCTCGTATGC212820.12699818352377293TruSeq Adapter, Index 1 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAGGG657550.055.03222370
CTGCTTG837150.054.87169657
TGCTTGA860800.053.34316358
CCGTCTT879000.052.6039150
TGCCGTC883750.052.52836648
GCTTGAA879500.052.48372359
GCCGTCT882500.052.33975649
ATGCCGT895000.051.82147647
TATGCCG900450.051.6619246
CGTCTTC920950.050.30611851
CACGATC831050.049.93781736
TCTGCTT921150.049.9057856
GTATGCC935950.049.762145
GATCTCG835700.049.67207339
CTTCTGC931650.049.67032254
ACGATCT843050.049.1273737
TCACGAT888600.048.74349635
CGATCTC851750.048.7036438
GTCTTCT950950.048.5718752
CTCGTAT921250.048.47007442
Produced by FastQC (version 0.11.2)