FastQCFastQC Report
Tue 29 May 2018
H3HF7AFXY_n01_triple-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3HF7AFXY_n01_triple-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13266463
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG1165020.8781692603371373TruSeq Adapter, Index 8 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCTTG239300.038.89167458
TGCCGTC260550.038.45959549
TATGCCG270750.038.24022747
ATGCCGT266550.038.18577648
GCCGTCT243600.037.7018450
GTATGCC284550.036.97540746
CCGTCTT256500.036.71914351
TGCTTGA262500.036.05426859
GCTTGAA285250.035.91477260
CGTATGC293950.035.126245
CTCGTAT276800.034.735543
ATCTCGT281650.033.62644641
GTCACAC395500.033.5548530
CGTCTTC279600.033.36001652
TCGTATG290450.033.1994744
AATCTCG297100.033.1246340
GAATCTC314350.031.91936739
AGTCACA419950.031.70946529
CAGTCAC421750.031.57329828
TCTCGTA301950.031.49444242
Produced by FastQC (version 0.11.2)