FastQCFastQC Report
Tue 29 May 2018
H3HF7AFXY_n01_rad27-exoi-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3HF7AFXY_n01_rad27-exoi-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13320070
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG590890.44360877983373964TruSeq Adapter, Index 6 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTGTG83100.024.3559071
ATGCCGG27400.023.37612770
CGTGTGC111700.021.5994911
CTGCTTG235750.019.31570858
GTGTGCT130500.018.7007482
TGCTTGA256600.017.95075259
GCTTGAA264950.017.66224960
CCGATCT275750.015.8595236
TCCGATC278450.015.730895
TATACGG45950.015.690762
TCTGCTT296950.015.60628257
CTTCTGC299750.015.37887955
CTTGAAA311950.015.34840861
CTTCCGA288500.015.2918423
TACGGTG46900.014.92562264
TCTTCCG301650.014.8574182
TAACTAG39300.014.78246330
CGATCTG110450.014.7341477
TTCCGAT300600.014.6647644
TGTGCTC168200.014.6132293
Produced by FastQC (version 0.11.2)