FastQCFastQC Report
Tue 29 May 2018
H3HF7AFXY_n01_rad27-dna2-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3HF7AFXY_n01_rad27-dna2-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11909093
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG720860.6053021838019067TruSeq Adapter, Index 5 (100% over 49bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGG37950.025.08561770
ACGTGTG128300.025.0015131
CTGCTTG256250.023.2063258
GACGTGT84600.022.724771
GCTTGAA288250.021.8074460
TGCTTGA273950.021.7707459
AAAAAGG234150.021.55453970
CGTGTGC179500.021.4598031
GTGTGCT197350.019.4805262
TCTGCTT313450.019.20640457
CTTGAAA343250.018.76150361
CTTCTGC319900.018.69911255
CCGTCTT349500.017.7253851
TTCTGCT330550.017.65146356
GCCGTCT353200.017.35163550
GTCTTCT344700.017.2719653
TGCCGTC373850.017.09529149
CGTCTTC369400.016.7610252
TGTGCTC233750.016.5517653
TCTTCTG361050.016.49015254
Produced by FastQC (version 0.11.2)