FastQCFastQC Report
Tue 29 May 2018
H3HF7AFXY_n01_rad27-asynch.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3HF7AFXY_n01_rad27-asynch.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11127878
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCTCGTATG1513491.360088599102183TruSeq Adapter, Index 18 (100% over 49bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTGAA1030100.040.8831866
ACGTGTG329150.040.8218841
CTTGAAA1047450.040.6998167
TGCTTGA1017600.040.1226865
CTGCTTG1017350.039.9393464
TGAAAAA1166750.039.44812469
GAAAAAA1198250.039.0973670
TTGAAAA1117800.038.51737668
TCTGCTT1070100.038.2050263
CTTCTGC1089400.038.1705561
TTCTGCT1066550.037.62975362
GTCTTCT1130800.036.21465359
TCTTCTG1140150.035.96000360
GACGTGT132750.034.2928771
ATGCCGG115650.034.2289470
CCGTCTT1264700.033.64797257
CGTCTTC1259600.033.3312758
AAAAAGG308250.032.80355570
GCGTGAA60400.032.4516966
GCCGTCT1302550.032.40135656
Produced by FastQC (version 0.11.2)