FastQCFastQC Report
Tue 29 May 2018
H3HF7AFXY_n01_rad27-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3HF7AFXY_n01_rad27-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15264799
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC4553702.983137871648359TruSeq Adapter, Index 2 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG438020.28694776786775905TruSeq Adapter, Index 2 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAGGG544700.053.83540770
CTGCTTG669850.051.0261157
TGCTTGA699500.049.05711458
GCCGTCT715650.048.78037649
CCGTCTT723750.048.42304650
TGCCGTC736650.048.37868548
GCTTGAA728550.048.037759
ATGCCGT751450.047.7062647
TATGCCG764950.047.19603746
CGTCTTC751500.046.49524751
TCTGCTT740200.046.47913756
CTTCTGC749050.046.32687854
GTATGCC794800.045.59935845
TATCTCG771600.044.881239
GTCTTCT771000.044.7427652
CGTATGC811750.044.4357944
TTCTGCT761750.044.35580455
TCTTCTG780100.044.26671253
CTCGTAT792850.044.1705842
CTTGAAA801050.044.07901460
Produced by FastQC (version 0.11.2)