FastQCFastQC Report
Tue 29 May 2018
H3HF7AFXY_n01_exoi-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3HF7AFXY_n01_exoi-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12514684
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC2203551.760771586402022TruSeq Adapter, Index 4 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG167600.13392267835128718TruSeq Adapter, Index 4 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCTTG361550.050.18637557
TGCTTGA383200.047.51460358
AAAAGGG303750.047.39282270
GCCGTCT391850.046.48340649
CCGTCTT394550.046.2715850
TGCCGTC397150.046.2686748
GCTTGAA398600.045.88083359
ATGCCGT407400.045.19871547
TATGCCG409650.044.9833846
TCTGCTT424000.042.92610556
CGTCTTC426150.042.8979351
GTATGCC432150.042.74659745
CTTCTGC434400.042.01960454
CGTATGC443900.041.6858944
CTCGTAT427000.041.22101642
GTCTTCT442350.041.20804652
TCGTATG451500.040.94544643
AATCTCG417300.040.8615839
ATCTCGT417750.040.7590840
GACCAAT436300.040.71793435
Produced by FastQC (version 0.11.2)