FastQCFastQC Report
Tue 29 May 2018
H3HF7AFXY_n01_dna2-exoi-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3HF7AFXY_n01_dna2-exoi-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15158613
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG312300.20602148758596844TruSeq Adapter, Index 7 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCTTG208200.021.83752664
ACGTGTG87700.020.682291
TGCTTGA233200.019.76687265
GCTTGAA250700.019.14115566
GACGTGT61900.018.8940161
CGTGTGC111200.017.7285021
ATGCCGG39850.017.47849770
CCGTCTT279250.017.19656457
CCGATCT255150.017.0424276
GCCGTCT282300.017.03551156
TCCGATC259150.016.8604355
TCTGCTT285600.016.27444863
TGCCGTC301100.016.21644455
CTTCCGA274150.016.1422023
CTTGAAA309300.015.93332267
ATGCCGT312250.015.80536654
CTTCTGC298650.015.70383761
TCTTCCG285500.015.6112522
CGATCTA97500.015.5780927
TATGCCG326050.015.41526453
Produced by FastQC (version 0.11.2)